Journal: Stem Cell Research & Therapy

Article Title: Tonsil-derived mesenchymal stem cells enhance allogeneic bone marrow engraftment via collagen IV degradation

doi: 10.1186/s13287-021-02414-6

Figure Lengend Snippet: MMP3 expression and secretion is increased in TMSCs compared to other MSCs. a Nano-liquid chromatography with tandem mass spectrometry analysis was performed using TMSC-CM, BMMSC-CM, and ADMSC-CM. Peptides of ECM-modulating enzymes are listed. Detected peptides are depicted in black. b The mRNA expression levels of MMP3, MMP1, CTSB, and PEPD in human TMSCs, BMMSCs, and ADMSCs were determined using real-time quantitative PCR (RT-qPCR) with custom oligos. GAPDH was used as the reference gene. c MMP3 protein expression levels in MSC lysates were determined by quantitative western blot analysis by dividing the pixel densities of the MMP3 bands by the pixel densities of the corresponding β-actin bands. d MMP3 protein concentrations in MSC-CM were quantified using a human MMP3 ELISA kit. The data represent the mean ± S.E.M. and were analyzed using one-way ANOVA (*P < 0.05)

Article Snippet: Bone marrow-derived MSCs (BMMSCs; PCS-500-012) were purchased from the ATCC (Manassas, VA, USA); adipose tissue-derived MSCs (ADMSCs; C-12977) and Wharton’s jelly-derived MSCs (WJMSCs; C-12971) were obtained from PromoCell (Heidelberg, Germany).

Techniques: Expressing, Liquid Chromatography, Mass Spectrometry, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Immunology

Article Title: Neutrophil depletion at the early stage of Japanese encephalitis virus infection affects CD8+ T cell infiltration into the mouse brain and causes severe encephalitis

doi: 10.3389/fimmu.2025.1748085

Figure Lengend Snippet: Chemokine levels in serum and brain lysates. (A) ELISA measured the concentration of CXCL12 (Top) and MIF-1 (bottom) levels in neutrophil-depleted mice, and AMD3100-treated infected mice serum (upper panel) and brain lysate (lower panel) plotted as a box plot. The concentration of MIF-1 level was measured using ELISA in neutrophil-depleted and AMD3100-treated infected mice serum (upper panel) and brain lysate (lower panel). (B) CXCL10 and CCL2 levels in serum (upper panel) and brain lysate (lower panel) in neutrophil-depleted mice and AMD3100-treated infected mice were measured by ELISA. The concentration of CXCL10 and CCL2 was plotted as a box plot. Each group has serum and brain lysate from (n=4) different infected mice. P - values were calculated using two-tailed unpaired Student’s t-test; *P<0.05; **P<0.01; ***P<0.001.

Article Snippet: The serum and brain lysate from infected mice (JEV-S1 & JEV-S3) were used to detect CXCL12 (E-EL-M3046; Elabscience, Texas, USA), MIF-1 (EM0440, Fine Test, Broadway Street, US), CXCL10 (EM0004, Fine Test, Broadway Street, US), and CCL2 (EM0135, Fine Test, Broadway Street, US).

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Infection, Two Tailed Test